Sterility:Article Title: Structural and immunogenetic signatures guide CD4-mimetic HIV vaccine development.
Article Snippet: .. N/A 001428 MD39.2 SOSIP Cottrell et al.46 N/A 20X HBS-EP+ Buffer, pH 7.6 (1 L, sterile) Teknova Cat# H8022 Water (Optima LC-MS grade) Fisher Scientific Cat# W6-4 IgG Elution Buffer Thermo Fisher Scientific Cat# 210099 Opti-MEM Thermo Fisher Scientific Cat# 31985-088 Bambanker Freezing Media BB01 Fisher Scientific (Bulldog Bio Inc.) Cat# 50-999-554 PEI MAX Transfection Grade Linear Polyethylenimine Hydrochloride (MW 40,000) Polysciences (Kyfora Bio) Cat# 24765 Human Capture Antibody Southern Biotech Cat# 2047-01 Endosafe Compendial LAL Cartridges, 0.05, 10/pk Fisher Scientific Cat# NC9594798 Phosphoric Acid 85 wt.% in H2O Thermo Fisher Scientific Cat# 345245-500ML Sodium Acetate pH 4.5, 10 mM Carterra Cat# 3622 Tween 20 Millipore Sigma Cat# P1379 Bovine Serum Albumin (BSA), Protease free Gold Biotechnologies Cat# A-420-10 PBS pH 7.4 (1X) Life Technologies Cat# 10010049 Tris-buffered saline (10X) Thermo Scientific Cat# J60764 rProtein A Sepharose Fast Flow resin Cytiva Cat# 17127903 FreeStyle 293 Expression Medium Thermo Fisher Scientific Cat# 12338026 PierceTM Protease Inhibitor Tablets, EDTAfree Thermo Fisher Scientific Cat# A32965 5M Sodium chloride (500 mL) Corning Cat# 46-032-CV 1M Tris-HCl, pH 8.0 solution. (1 L, Sterile) Teknova Cat# T1080 Bovine Serum Albumin (BSA) Fisher (Rockland) Cat# NC0263771 Ampicillin Sodium Salt (Crystalline Powder) Fisher Scientific Cat# BP1760-25 Carterra LSA Sensor Chip (CMDP) Carterra Cat# 4282 Amine Coupling Kit Cytiva Cat# BR100050 BiacoreTM Maintenance Kit, type 3 Cytiva Cat# 29229054 Dialyzer Midi VWR Cat# 71506-3 Lauryl Maltose Neopentyl Glycol (LMNG) Anatrace Cat# NG310 Penicillin-Streptomycin (10′000 U/mL) Thermo Fisher Scientific Cat# 15140122 L-Glutamine (200 mM) Thermo Fisher Scientific Cat# 25030081 Dulbecco’s Modified Eagle Medium (DMEM) Thermo Fisher Scientific Cat# 10313039 Heat Inactivated FBS Omega Scientific Cat# FB-02 (Continued on next page) Cell Reports 45, 117180, April 28, 2026 23 .. REAGENT or RESOURCE SOURCE IDENTIFIER FuGENE 6 Transfection Reagent FuGENE Inc. Cat# F6-5000 DEAE-Dextran Thermo Fisher Scientific Cat# 9015-73-0 Trypsin-EDTA (0.25%), phenol red Thermo Fisher Scientific Cat# 25200056 1X Dulbecco’s Phosphate-Buffered Saline (DPBS) Corning Cat# 21-031-CV Streptavidin AF647 Invitrogen Cat# S11223 Streptavidin-AF488 Invitrogen Cat# S21374 Streptavidin-BV421 Biolegend Cat# 405225 BD FC Beads BV510 BD Biosciences Cat# 661628 BD FC Beads APC-H7 BD Biosciences Cat# 661621 BD FC Beads APC-Cy7 BD Biosciences Cat# 661622 BD FC Beads PE BD Biosciences Cat# 661616 BD FC Beads PerCP Cy 5.5 BD Biosciences Cat# 661619 BD FC Beads BV786 BD Biosciences Cat# 661630 BD FC Beads PE Cy7 BD Biosciences Cat# 661617 BD FC Beads FITC BD Biosciences Cat# 661615 BD FC Beads APC BD Biosciences Cat# 661620 BD FC Beads BV421 BD Biosciences Cat# 661627 BD Resuspension Buffer (35mL) BD Biosciences Cat# 661614 Spherotech Anti-Human IgG/Protein Beads Spherotech Cat# HUP1505 Anti-Mouse Ig, κ/Negative Comp Beads BD Biosciences Cat# 552843 Mouse anti-human IgG BD Biosciences Cat# 555784 UltraComp Beads Plus Compensation Beads Thermo Fisher Scientific Cat# 01-333-42 ArC Reactive Beads Invitrogen Cat# A10346 Biotin Polystyrene Particles Spherotech Cat# TP-30-5 Glycerol, molecular grade Ricca Chemical Company Cat# 3290-32 Buffer EB Qiagen Cat# 19086 Ethanol, 100 Proof Pharmco Cat# 111000200 SPRI Beads Beckman Coulter Cat# B23319 Dynabeads Invitrogen Cat# 37002D Ambion Nuclease-free Water Invitrogen Cat# AM9937 RPMI Gibco Cat# 61870-036 EDTA Invitrogen Cat# 15575-038 CS&T Beads BD Biosciences Cat# 661414 Accudrop beads BD Biosciences Cat# 661612 Sheath Fluid BD Biosciences Cat# 99-30211-00 High Sensitivity D5000 ScreenTape Agilent Cat# 5067-5592 High Sensitivity D5000 Reagents Agilent Cat# 5067-5593 High Sensitivity D1000 ScreenTape Agilent Cat# 5067-5584 High Sensitivity D1000 Reagents Agilent Cat# 5067-5585 PhiX Control Library NextSeq V3 Illumina Cat# FC-110-3002 NextSeq 2000 P3 Reagents (100 Cycles) Illumina Cat# 20040559 Bovine Serum Albumin 30% Solution Fisher (Rockland) Cat# NC0263771 Microdialysis Plate 48 Wells 1 mL Thermo Fisher Scientific Car# A50466 Deposited data BG505 MD39 + RM19R Fab + 1–23 Fab model PDB: 9PIT This manuscript. (Continued on next page) 24 Cell Reports 45, 117180, April 28, 2026
Liquid Chromatography with Mass Spectroscopy:Article Title: Structural and immunogenetic signatures guide CD4-mimetic HIV vaccine development.
Article Snippet: .. N/A 001428 MD39.2 SOSIP Cottrell et al.46 N/A 20X HBS-EP+ Buffer, pH 7.6 (1 L, sterile) Teknova Cat# H8022 Water (Optima LC-MS grade) Fisher Scientific Cat# W6-4 IgG Elution Buffer Thermo Fisher Scientific Cat# 210099 Opti-MEM Thermo Fisher Scientific Cat# 31985-088 Bambanker Freezing Media BB01 Fisher Scientific (Bulldog Bio Inc.) Cat# 50-999-554 PEI MAX Transfection Grade Linear Polyethylenimine Hydrochloride (MW 40,000) Polysciences (Kyfora Bio) Cat# 24765 Human Capture Antibody Southern Biotech Cat# 2047-01 Endosafe Compendial LAL Cartridges, 0.05, 10/pk Fisher Scientific Cat# NC9594798 Phosphoric Acid 85 wt.% in H2O Thermo Fisher Scientific Cat# 345245-500ML Sodium Acetate pH 4.5, 10 mM Carterra Cat# 3622 Tween 20 Millipore Sigma Cat# P1379 Bovine Serum Albumin (BSA), Protease free Gold Biotechnologies Cat# A-420-10 PBS pH 7.4 (1X) Life Technologies Cat# 10010049 Tris-buffered saline (10X) Thermo Scientific Cat# J60764 rProtein A Sepharose Fast Flow resin Cytiva Cat# 17127903 FreeStyle 293 Expression Medium Thermo Fisher Scientific Cat# 12338026 PierceTM Protease Inhibitor Tablets, EDTAfree Thermo Fisher Scientific Cat# A32965 5M Sodium chloride (500 mL) Corning Cat# 46-032-CV 1M Tris-HCl, pH 8.0 solution. (1 L, Sterile) Teknova Cat# T1080 Bovine Serum Albumin (BSA) Fisher (Rockland) Cat# NC0263771 Ampicillin Sodium Salt (Crystalline Powder) Fisher Scientific Cat# BP1760-25 Carterra LSA Sensor Chip (CMDP) Carterra Cat# 4282 Amine Coupling Kit Cytiva Cat# BR100050 BiacoreTM Maintenance Kit, type 3 Cytiva Cat# 29229054 Dialyzer Midi VWR Cat# 71506-3 Lauryl Maltose Neopentyl Glycol (LMNG) Anatrace Cat# NG310 Penicillin-Streptomycin (10′000 U/mL) Thermo Fisher Scientific Cat# 15140122 L-Glutamine (200 mM) Thermo Fisher Scientific Cat# 25030081 Dulbecco’s Modified Eagle Medium (DMEM) Thermo Fisher Scientific Cat# 10313039 Heat Inactivated FBS Omega Scientific Cat# FB-02 (Continued on next page) Cell Reports 45, 117180, April 28, 2026 23 .. REAGENT or RESOURCE SOURCE IDENTIFIER FuGENE 6 Transfection Reagent FuGENE Inc. Cat# F6-5000 DEAE-Dextran Thermo Fisher Scientific Cat# 9015-73-0 Trypsin-EDTA (0.25%), phenol red Thermo Fisher Scientific Cat# 25200056 1X Dulbecco’s Phosphate-Buffered Saline (DPBS) Corning Cat# 21-031-CV Streptavidin AF647 Invitrogen Cat# S11223 Streptavidin-AF488 Invitrogen Cat# S21374 Streptavidin-BV421 Biolegend Cat# 405225 BD FC Beads BV510 BD Biosciences Cat# 661628 BD FC Beads APC-H7 BD Biosciences Cat# 661621 BD FC Beads APC-Cy7 BD Biosciences Cat# 661622 BD FC Beads PE BD Biosciences Cat# 661616 BD FC Beads PerCP Cy 5.5 BD Biosciences Cat# 661619 BD FC Beads BV786 BD Biosciences Cat# 661630 BD FC Beads PE Cy7 BD Biosciences Cat# 661617 BD FC Beads FITC BD Biosciences Cat# 661615 BD FC Beads APC BD Biosciences Cat# 661620 BD FC Beads BV421 BD Biosciences Cat# 661627 BD Resuspension Buffer (35mL) BD Biosciences Cat# 661614 Spherotech Anti-Human IgG/Protein Beads Spherotech Cat# HUP1505 Anti-Mouse Ig, κ/Negative Comp Beads BD Biosciences Cat# 552843 Mouse anti-human IgG BD Biosciences Cat# 555784 UltraComp Beads Plus Compensation Beads Thermo Fisher Scientific Cat# 01-333-42 ArC Reactive Beads Invitrogen Cat# A10346 Biotin Polystyrene Particles Spherotech Cat# TP-30-5 Glycerol, molecular grade Ricca Chemical Company Cat# 3290-32 Buffer EB Qiagen Cat# 19086 Ethanol, 100 Proof Pharmco Cat# 111000200 SPRI Beads Beckman Coulter Cat# B23319 Dynabeads Invitrogen Cat# 37002D Ambion Nuclease-free Water Invitrogen Cat# AM9937 RPMI Gibco Cat# 61870-036 EDTA Invitrogen Cat# 15575-038 CS&T Beads BD Biosciences Cat# 661414 Accudrop beads BD Biosciences Cat# 661612 Sheath Fluid BD Biosciences Cat# 99-30211-00 High Sensitivity D5000 ScreenTape Agilent Cat# 5067-5592 High Sensitivity D5000 Reagents Agilent Cat# 5067-5593 High Sensitivity D1000 ScreenTape Agilent Cat# 5067-5584 High Sensitivity D1000 Reagents Agilent Cat# 5067-5585 PhiX Control Library NextSeq V3 Illumina Cat# FC-110-3002 NextSeq 2000 P3 Reagents (100 Cycles) Illumina Cat# 20040559 Bovine Serum Albumin 30% Solution Fisher (Rockland) Cat# NC0263771 Microdialysis Plate 48 Wells 1 mL Thermo Fisher Scientific Car# A50466 Deposited data BG505 MD39 + RM19R Fab + 1–23 Fab model PDB: 9PIT This manuscript. (Continued on next page) 24 Cell Reports 45, 117180, April 28, 2026
Transfection:Article Title: Structural and immunogenetic signatures guide CD4-mimetic HIV vaccine development.
Article Snippet: .. N/A 001428 MD39.2 SOSIP Cottrell et al.46 N/A 20X HBS-EP+ Buffer, pH 7.6 (1 L, sterile) Teknova Cat# H8022 Water (Optima LC-MS grade) Fisher Scientific Cat# W6-4 IgG Elution Buffer Thermo Fisher Scientific Cat# 210099 Opti-MEM Thermo Fisher Scientific Cat# 31985-088 Bambanker Freezing Media BB01 Fisher Scientific (Bulldog Bio Inc.) Cat# 50-999-554 PEI MAX Transfection Grade Linear Polyethylenimine Hydrochloride (MW 40,000) Polysciences (Kyfora Bio) Cat# 24765 Human Capture Antibody Southern Biotech Cat# 2047-01 Endosafe Compendial LAL Cartridges, 0.05, 10/pk Fisher Scientific Cat# NC9594798 Phosphoric Acid 85 wt.% in H2O Thermo Fisher Scientific Cat# 345245-500ML Sodium Acetate pH 4.5, 10 mM Carterra Cat# 3622 Tween 20 Millipore Sigma Cat# P1379 Bovine Serum Albumin (BSA), Protease free Gold Biotechnologies Cat# A-420-10 PBS pH 7.4 (1X) Life Technologies Cat# 10010049 Tris-buffered saline (10X) Thermo Scientific Cat# J60764 rProtein A Sepharose Fast Flow resin Cytiva Cat# 17127903 FreeStyle 293 Expression Medium Thermo Fisher Scientific Cat# 12338026 PierceTM Protease Inhibitor Tablets, EDTAfree Thermo Fisher Scientific Cat# A32965 5M Sodium chloride (500 mL) Corning Cat# 46-032-CV 1M Tris-HCl, pH 8.0 solution. (1 L, Sterile) Teknova Cat# T1080 Bovine Serum Albumin (BSA) Fisher (Rockland) Cat# NC0263771 Ampicillin Sodium Salt (Crystalline Powder) Fisher Scientific Cat# BP1760-25 Carterra LSA Sensor Chip (CMDP) Carterra Cat# 4282 Amine Coupling Kit Cytiva Cat# BR100050 BiacoreTM Maintenance Kit, type 3 Cytiva Cat# 29229054 Dialyzer Midi VWR Cat# 71506-3 Lauryl Maltose Neopentyl Glycol (LMNG) Anatrace Cat# NG310 Penicillin-Streptomycin (10′000 U/mL) Thermo Fisher Scientific Cat# 15140122 L-Glutamine (200 mM) Thermo Fisher Scientific Cat# 25030081 Dulbecco’s Modified Eagle Medium (DMEM) Thermo Fisher Scientific Cat# 10313039 Heat Inactivated FBS Omega Scientific Cat# FB-02 (Continued on next page) Cell Reports 45, 117180, April 28, 2026 23 .. REAGENT or RESOURCE SOURCE IDENTIFIER FuGENE 6 Transfection Reagent FuGENE Inc. Cat# F6-5000 DEAE-Dextran Thermo Fisher Scientific Cat# 9015-73-0 Trypsin-EDTA (0.25%), phenol red Thermo Fisher Scientific Cat# 25200056 1X Dulbecco’s Phosphate-Buffered Saline (DPBS) Corning Cat# 21-031-CV Streptavidin AF647 Invitrogen Cat# S11223 Streptavidin-AF488 Invitrogen Cat# S21374 Streptavidin-BV421 Biolegend Cat# 405225 BD FC Beads BV510 BD Biosciences Cat# 661628 BD FC Beads APC-H7 BD Biosciences Cat# 661621 BD FC Beads APC-Cy7 BD Biosciences Cat# 661622 BD FC Beads PE BD Biosciences Cat# 661616 BD FC Beads PerCP Cy 5.5 BD Biosciences Cat# 661619 BD FC Beads BV786 BD Biosciences Cat# 661630 BD FC Beads PE Cy7 BD Biosciences Cat# 661617 BD FC Beads FITC BD Biosciences Cat# 661615 BD FC Beads APC BD Biosciences Cat# 661620 BD FC Beads BV421 BD Biosciences Cat# 661627 BD Resuspension Buffer (35mL) BD Biosciences Cat# 661614 Spherotech Anti-Human IgG/Protein Beads Spherotech Cat# HUP1505 Anti-Mouse Ig, κ/Negative Comp Beads BD Biosciences Cat# 552843 Mouse anti-human IgG BD Biosciences Cat# 555784 UltraComp Beads Plus Compensation Beads Thermo Fisher Scientific Cat# 01-333-42 ArC Reactive Beads Invitrogen Cat# A10346 Biotin Polystyrene Particles Spherotech Cat# TP-30-5 Glycerol, molecular grade Ricca Chemical Company Cat# 3290-32 Buffer EB Qiagen Cat# 19086 Ethanol, 100 Proof Pharmco Cat# 111000200 SPRI Beads Beckman Coulter Cat# B23319 Dynabeads Invitrogen Cat# 37002D Ambion Nuclease-free Water Invitrogen Cat# AM9937 RPMI Gibco Cat# 61870-036 EDTA Invitrogen Cat# 15575-038 CS&T Beads BD Biosciences Cat# 661414 Accudrop beads BD Biosciences Cat# 661612 Sheath Fluid BD Biosciences Cat# 99-30211-00 High Sensitivity D5000 ScreenTape Agilent Cat# 5067-5592 High Sensitivity D5000 Reagents Agilent Cat# 5067-5593 High Sensitivity D1000 ScreenTape Agilent Cat# 5067-5584 High Sensitivity D1000 Reagents Agilent Cat# 5067-5585 PhiX Control Library NextSeq V3 Illumina Cat# FC-110-3002 NextSeq 2000 P3 Reagents (100 Cycles) Illumina Cat# 20040559 Bovine Serum Albumin 30% Solution Fisher (Rockland) Cat# NC0263771 Microdialysis Plate 48 Wells 1 mL Thermo Fisher Scientific Car# A50466 Deposited data BG505 MD39 + RM19R Fab + 1–23 Fab model PDB: 9PIT This manuscript. (Continued on next page) 24 Cell Reports 45, 117180, April 28, 2026
Saline:Article Title: Structural and immunogenetic signatures guide CD4-mimetic HIV vaccine development.
Article Snippet: .. N/A 001428 MD39.2 SOSIP Cottrell et al.46 N/A 20X HBS-EP+ Buffer, pH 7.6 (1 L, sterile) Teknova Cat# H8022 Water (Optima LC-MS grade) Fisher Scientific Cat# W6-4 IgG Elution Buffer Thermo Fisher Scientific Cat# 210099 Opti-MEM Thermo Fisher Scientific Cat# 31985-088 Bambanker Freezing Media BB01 Fisher Scientific (Bulldog Bio Inc.) Cat# 50-999-554 PEI MAX Transfection Grade Linear Polyethylenimine Hydrochloride (MW 40,000) Polysciences (Kyfora Bio) Cat# 24765 Human Capture Antibody Southern Biotech Cat# 2047-01 Endosafe Compendial LAL Cartridges, 0.05, 10/pk Fisher Scientific Cat# NC9594798 Phosphoric Acid 85 wt.% in H2O Thermo Fisher Scientific Cat# 345245-500ML Sodium Acetate pH 4.5, 10 mM Carterra Cat# 3622 Tween 20 Millipore Sigma Cat# P1379 Bovine Serum Albumin (BSA), Protease free Gold Biotechnologies Cat# A-420-10 PBS pH 7.4 (1X) Life Technologies Cat# 10010049 Tris-buffered saline (10X) Thermo Scientific Cat# J60764 rProtein A Sepharose Fast Flow resin Cytiva Cat# 17127903 FreeStyle 293 Expression Medium Thermo Fisher Scientific Cat# 12338026 PierceTM Protease Inhibitor Tablets, EDTAfree Thermo Fisher Scientific Cat# A32965 5M Sodium chloride (500 mL) Corning Cat# 46-032-CV 1M Tris-HCl, pH 8.0 solution. (1 L, Sterile) Teknova Cat# T1080 Bovine Serum Albumin (BSA) Fisher (Rockland) Cat# NC0263771 Ampicillin Sodium Salt (Crystalline Powder) Fisher Scientific Cat# BP1760-25 Carterra LSA Sensor Chip (CMDP) Carterra Cat# 4282 Amine Coupling Kit Cytiva Cat# BR100050 BiacoreTM Maintenance Kit, type 3 Cytiva Cat# 29229054 Dialyzer Midi VWR Cat# 71506-3 Lauryl Maltose Neopentyl Glycol (LMNG) Anatrace Cat# NG310 Penicillin-Streptomycin (10′000 U/mL) Thermo Fisher Scientific Cat# 15140122 L-Glutamine (200 mM) Thermo Fisher Scientific Cat# 25030081 Dulbecco’s Modified Eagle Medium (DMEM) Thermo Fisher Scientific Cat# 10313039 Heat Inactivated FBS Omega Scientific Cat# FB-02 (Continued on next page) Cell Reports 45, 117180, April 28, 2026 23 .. REAGENT or RESOURCE SOURCE IDENTIFIER FuGENE 6 Transfection Reagent FuGENE Inc. Cat# F6-5000 DEAE-Dextran Thermo Fisher Scientific Cat# 9015-73-0 Trypsin-EDTA (0.25%), phenol red Thermo Fisher Scientific Cat# 25200056 1X Dulbecco’s Phosphate-Buffered Saline (DPBS) Corning Cat# 21-031-CV Streptavidin AF647 Invitrogen Cat# S11223 Streptavidin-AF488 Invitrogen Cat# S21374 Streptavidin-BV421 Biolegend Cat# 405225 BD FC Beads BV510 BD Biosciences Cat# 661628 BD FC Beads APC-H7 BD Biosciences Cat# 661621 BD FC Beads APC-Cy7 BD Biosciences Cat# 661622 BD FC Beads PE BD Biosciences Cat# 661616 BD FC Beads PerCP Cy 5.5 BD Biosciences Cat# 661619 BD FC Beads BV786 BD Biosciences Cat# 661630 BD FC Beads PE Cy7 BD Biosciences Cat# 661617 BD FC Beads FITC BD Biosciences Cat# 661615 BD FC Beads APC BD Biosciences Cat# 661620 BD FC Beads BV421 BD Biosciences Cat# 661627 BD Resuspension Buffer (35mL) BD Biosciences Cat# 661614 Spherotech Anti-Human IgG/Protein Beads Spherotech Cat# HUP1505 Anti-Mouse Ig, κ/Negative Comp Beads BD Biosciences Cat# 552843 Mouse anti-human IgG BD Biosciences Cat# 555784 UltraComp Beads Plus Compensation Beads Thermo Fisher Scientific Cat# 01-333-42 ArC Reactive Beads Invitrogen Cat# A10346 Biotin Polystyrene Particles Spherotech Cat# TP-30-5 Glycerol, molecular grade Ricca Chemical Company Cat# 3290-32 Buffer EB Qiagen Cat# 19086 Ethanol, 100 Proof Pharmco Cat# 111000200 SPRI Beads Beckman Coulter Cat# B23319 Dynabeads Invitrogen Cat# 37002D Ambion Nuclease-free Water Invitrogen Cat# AM9937 RPMI Gibco Cat# 61870-036 EDTA Invitrogen Cat# 15575-038 CS&T Beads BD Biosciences Cat# 661414 Accudrop beads BD Biosciences Cat# 661612 Sheath Fluid BD Biosciences Cat# 99-30211-00 High Sensitivity D5000 ScreenTape Agilent Cat# 5067-5592 High Sensitivity D5000 Reagents Agilent Cat# 5067-5593 High Sensitivity D1000 ScreenTape Agilent Cat# 5067-5584 High Sensitivity D1000 Reagents Agilent Cat# 5067-5585 PhiX Control Library NextSeq V3 Illumina Cat# FC-110-3002 NextSeq 2000 P3 Reagents (100 Cycles) Illumina Cat# 20040559 Bovine Serum Albumin 30% Solution Fisher (Rockland) Cat# NC0263771 Microdialysis Plate 48 Wells 1 mL Thermo Fisher Scientific Car# A50466 Deposited data BG505 MD39 + RM19R Fab + 1–23 Fab model PDB: 9PIT This manuscript. (Continued on next page) 24 Cell Reports 45, 117180, April 28, 2026
Expressing:Article Title: Structural and immunogenetic signatures guide CD4-mimetic HIV vaccine development.
Article Snippet: .. N/A 001428 MD39.2 SOSIP Cottrell et al.46 N/A 20X HBS-EP+ Buffer, pH 7.6 (1 L, sterile) Teknova Cat# H8022 Water (Optima LC-MS grade) Fisher Scientific Cat# W6-4 IgG Elution Buffer Thermo Fisher Scientific Cat# 210099 Opti-MEM Thermo Fisher Scientific Cat# 31985-088 Bambanker Freezing Media BB01 Fisher Scientific (Bulldog Bio Inc.) Cat# 50-999-554 PEI MAX Transfection Grade Linear Polyethylenimine Hydrochloride (MW 40,000) Polysciences (Kyfora Bio) Cat# 24765 Human Capture Antibody Southern Biotech Cat# 2047-01 Endosafe Compendial LAL Cartridges, 0.05, 10/pk Fisher Scientific Cat# NC9594798 Phosphoric Acid 85 wt.% in H2O Thermo Fisher Scientific Cat# 345245-500ML Sodium Acetate pH 4.5, 10 mM Carterra Cat# 3622 Tween 20 Millipore Sigma Cat# P1379 Bovine Serum Albumin (BSA), Protease free Gold Biotechnologies Cat# A-420-10 PBS pH 7.4 (1X) Life Technologies Cat# 10010049 Tris-buffered saline (10X) Thermo Scientific Cat# J60764 rProtein A Sepharose Fast Flow resin Cytiva Cat# 17127903 FreeStyle 293 Expression Medium Thermo Fisher Scientific Cat# 12338026 PierceTM Protease Inhibitor Tablets, EDTAfree Thermo Fisher Scientific Cat# A32965 5M Sodium chloride (500 mL) Corning Cat# 46-032-CV 1M Tris-HCl, pH 8.0 solution. (1 L, Sterile) Teknova Cat# T1080 Bovine Serum Albumin (BSA) Fisher (Rockland) Cat# NC0263771 Ampicillin Sodium Salt (Crystalline Powder) Fisher Scientific Cat# BP1760-25 Carterra LSA Sensor Chip (CMDP) Carterra Cat# 4282 Amine Coupling Kit Cytiva Cat# BR100050 BiacoreTM Maintenance Kit, type 3 Cytiva Cat# 29229054 Dialyzer Midi VWR Cat# 71506-3 Lauryl Maltose Neopentyl Glycol (LMNG) Anatrace Cat# NG310 Penicillin-Streptomycin (10′000 U/mL) Thermo Fisher Scientific Cat# 15140122 L-Glutamine (200 mM) Thermo Fisher Scientific Cat# 25030081 Dulbecco’s Modified Eagle Medium (DMEM) Thermo Fisher Scientific Cat# 10313039 Heat Inactivated FBS Omega Scientific Cat# FB-02 (Continued on next page) Cell Reports 45, 117180, April 28, 2026 23 .. REAGENT or RESOURCE SOURCE IDENTIFIER FuGENE 6 Transfection Reagent FuGENE Inc. Cat# F6-5000 DEAE-Dextran Thermo Fisher Scientific Cat# 9015-73-0 Trypsin-EDTA (0.25%), phenol red Thermo Fisher Scientific Cat# 25200056 1X Dulbecco’s Phosphate-Buffered Saline (DPBS) Corning Cat# 21-031-CV Streptavidin AF647 Invitrogen Cat# S11223 Streptavidin-AF488 Invitrogen Cat# S21374 Streptavidin-BV421 Biolegend Cat# 405225 BD FC Beads BV510 BD Biosciences Cat# 661628 BD FC Beads APC-H7 BD Biosciences Cat# 661621 BD FC Beads APC-Cy7 BD Biosciences Cat# 661622 BD FC Beads PE BD Biosciences Cat# 661616 BD FC Beads PerCP Cy 5.5 BD Biosciences Cat# 661619 BD FC Beads BV786 BD Biosciences Cat# 661630 BD FC Beads PE Cy7 BD Biosciences Cat# 661617 BD FC Beads FITC BD Biosciences Cat# 661615 BD FC Beads APC BD Biosciences Cat# 661620 BD FC Beads BV421 BD Biosciences Cat# 661627 BD Resuspension Buffer (35mL) BD Biosciences Cat# 661614 Spherotech Anti-Human IgG/Protein Beads Spherotech Cat# HUP1505 Anti-Mouse Ig, κ/Negative Comp Beads BD Biosciences Cat# 552843 Mouse anti-human IgG BD Biosciences Cat# 555784 UltraComp Beads Plus Compensation Beads Thermo Fisher Scientific Cat# 01-333-42 ArC Reactive Beads Invitrogen Cat# A10346 Biotin Polystyrene Particles Spherotech Cat# TP-30-5 Glycerol, molecular grade Ricca Chemical Company Cat# 3290-32 Buffer EB Qiagen Cat# 19086 Ethanol, 100 Proof Pharmco Cat# 111000200 SPRI Beads Beckman Coulter Cat# B23319 Dynabeads Invitrogen Cat# 37002D Ambion Nuclease-free Water Invitrogen Cat# AM9937 RPMI Gibco Cat# 61870-036 EDTA Invitrogen Cat# 15575-038 CS&T Beads BD Biosciences Cat# 661414 Accudrop beads BD Biosciences Cat# 661612 Sheath Fluid BD Biosciences Cat# 99-30211-00 High Sensitivity D5000 ScreenTape Agilent Cat# 5067-5592 High Sensitivity D5000 Reagents Agilent Cat# 5067-5593 High Sensitivity D1000 ScreenTape Agilent Cat# 5067-5584 High Sensitivity D1000 Reagents Agilent Cat# 5067-5585 PhiX Control Library NextSeq V3 Illumina Cat# FC-110-3002 NextSeq 2000 P3 Reagents (100 Cycles) Illumina Cat# 20040559 Bovine Serum Albumin 30% Solution Fisher (Rockland) Cat# NC0263771 Microdialysis Plate 48 Wells 1 mL Thermo Fisher Scientific Car# A50466 Deposited data BG505 MD39 + RM19R Fab + 1–23 Fab model PDB: 9PIT This manuscript. (Continued on next page) 24 Cell Reports 45, 117180, April 28, 2026
Protease Inhibitor:Article Title: Structural and immunogenetic signatures guide CD4-mimetic HIV vaccine development.
Article Snippet: .. N/A 001428 MD39.2 SOSIP Cottrell et al.46 N/A 20X HBS-EP+ Buffer, pH 7.6 (1 L, sterile) Teknova Cat# H8022 Water (Optima LC-MS grade) Fisher Scientific Cat# W6-4 IgG Elution Buffer Thermo Fisher Scientific Cat# 210099 Opti-MEM Thermo Fisher Scientific Cat# 31985-088 Bambanker Freezing Media BB01 Fisher Scientific (Bulldog Bio Inc.) Cat# 50-999-554 PEI MAX Transfection Grade Linear Polyethylenimine Hydrochloride (MW 40,000) Polysciences (Kyfora Bio) Cat# 24765 Human Capture Antibody Southern Biotech Cat# 2047-01 Endosafe Compendial LAL Cartridges, 0.05, 10/pk Fisher Scientific Cat# NC9594798 Phosphoric Acid 85 wt.% in H2O Thermo Fisher Scientific Cat# 345245-500ML Sodium Acetate pH 4.5, 10 mM Carterra Cat# 3622 Tween 20 Millipore Sigma Cat# P1379 Bovine Serum Albumin (BSA), Protease free Gold Biotechnologies Cat# A-420-10 PBS pH 7.4 (1X) Life Technologies Cat# 10010049 Tris-buffered saline (10X) Thermo Scientific Cat# J60764 rProtein A Sepharose Fast Flow resin Cytiva Cat# 17127903 FreeStyle 293 Expression Medium Thermo Fisher Scientific Cat# 12338026 PierceTM Protease Inhibitor Tablets, EDTAfree Thermo Fisher Scientific Cat# A32965 5M Sodium chloride (500 mL) Corning Cat# 46-032-CV 1M Tris-HCl, pH 8.0 solution. (1 L, Sterile) Teknova Cat# T1080 Bovine Serum Albumin (BSA) Fisher (Rockland) Cat# NC0263771 Ampicillin Sodium Salt (Crystalline Powder) Fisher Scientific Cat# BP1760-25 Carterra LSA Sensor Chip (CMDP) Carterra Cat# 4282 Amine Coupling Kit Cytiva Cat# BR100050 BiacoreTM Maintenance Kit, type 3 Cytiva Cat# 29229054 Dialyzer Midi VWR Cat# 71506-3 Lauryl Maltose Neopentyl Glycol (LMNG) Anatrace Cat# NG310 Penicillin-Streptomycin (10′000 U/mL) Thermo Fisher Scientific Cat# 15140122 L-Glutamine (200 mM) Thermo Fisher Scientific Cat# 25030081 Dulbecco’s Modified Eagle Medium (DMEM) Thermo Fisher Scientific Cat# 10313039 Heat Inactivated FBS Omega Scientific Cat# FB-02 (Continued on next page) Cell Reports 45, 117180, April 28, 2026 23 .. REAGENT or RESOURCE SOURCE IDENTIFIER FuGENE 6 Transfection Reagent FuGENE Inc. Cat# F6-5000 DEAE-Dextran Thermo Fisher Scientific Cat# 9015-73-0 Trypsin-EDTA (0.25%), phenol red Thermo Fisher Scientific Cat# 25200056 1X Dulbecco’s Phosphate-Buffered Saline (DPBS) Corning Cat# 21-031-CV Streptavidin AF647 Invitrogen Cat# S11223 Streptavidin-AF488 Invitrogen Cat# S21374 Streptavidin-BV421 Biolegend Cat# 405225 BD FC Beads BV510 BD Biosciences Cat# 661628 BD FC Beads APC-H7 BD Biosciences Cat# 661621 BD FC Beads APC-Cy7 BD Biosciences Cat# 661622 BD FC Beads PE BD Biosciences Cat# 661616 BD FC Beads PerCP Cy 5.5 BD Biosciences Cat# 661619 BD FC Beads BV786 BD Biosciences Cat# 661630 BD FC Beads PE Cy7 BD Biosciences Cat# 661617 BD FC Beads FITC BD Biosciences Cat# 661615 BD FC Beads APC BD Biosciences Cat# 661620 BD FC Beads BV421 BD Biosciences Cat# 661627 BD Resuspension Buffer (35mL) BD Biosciences Cat# 661614 Spherotech Anti-Human IgG/Protein Beads Spherotech Cat# HUP1505 Anti-Mouse Ig, κ/Negative Comp Beads BD Biosciences Cat# 552843 Mouse anti-human IgG BD Biosciences Cat# 555784 UltraComp Beads Plus Compensation Beads Thermo Fisher Scientific Cat# 01-333-42 ArC Reactive Beads Invitrogen Cat# A10346 Biotin Polystyrene Particles Spherotech Cat# TP-30-5 Glycerol, molecular grade Ricca Chemical Company Cat# 3290-32 Buffer EB Qiagen Cat# 19086 Ethanol, 100 Proof Pharmco Cat# 111000200 SPRI Beads Beckman Coulter Cat# B23319 Dynabeads Invitrogen Cat# 37002D Ambion Nuclease-free Water Invitrogen Cat# AM9937 RPMI Gibco Cat# 61870-036 EDTA Invitrogen Cat# 15575-038 CS&T Beads BD Biosciences Cat# 661414 Accudrop beads BD Biosciences Cat# 661612 Sheath Fluid BD Biosciences Cat# 99-30211-00 High Sensitivity D5000 ScreenTape Agilent Cat# 5067-5592 High Sensitivity D5000 Reagents Agilent Cat# 5067-5593 High Sensitivity D1000 ScreenTape Agilent Cat# 5067-5584 High Sensitivity D1000 Reagents Agilent Cat# 5067-5585 PhiX Control Library NextSeq V3 Illumina Cat# FC-110-3002 NextSeq 2000 P3 Reagents (100 Cycles) Illumina Cat# 20040559 Bovine Serum Albumin 30% Solution Fisher (Rockland) Cat# NC0263771 Microdialysis Plate 48 Wells 1 mL Thermo Fisher Scientific Car# A50466 Deposited data BG505 MD39 + RM19R Fab + 1–23 Fab model PDB: 9PIT This manuscript. (Continued on next page) 24 Cell Reports 45, 117180, April 28, 2026
Modification:Article Title: Structural and immunogenetic signatures guide CD4-mimetic HIV vaccine development.
Article Snippet: .. N/A 001428 MD39.2 SOSIP Cottrell et al.46 N/A 20X HBS-EP+ Buffer, pH 7.6 (1 L, sterile) Teknova Cat# H8022 Water (Optima LC-MS grade) Fisher Scientific Cat# W6-4 IgG Elution Buffer Thermo Fisher Scientific Cat# 210099 Opti-MEM Thermo Fisher Scientific Cat# 31985-088 Bambanker Freezing Media BB01 Fisher Scientific (Bulldog Bio Inc.) Cat# 50-999-554 PEI MAX Transfection Grade Linear Polyethylenimine Hydrochloride (MW 40,000) Polysciences (Kyfora Bio) Cat# 24765 Human Capture Antibody Southern Biotech Cat# 2047-01 Endosafe Compendial LAL Cartridges, 0.05, 10/pk Fisher Scientific Cat# NC9594798 Phosphoric Acid 85 wt.% in H2O Thermo Fisher Scientific Cat# 345245-500ML Sodium Acetate pH 4.5, 10 mM Carterra Cat# 3622 Tween 20 Millipore Sigma Cat# P1379 Bovine Serum Albumin (BSA), Protease free Gold Biotechnologies Cat# A-420-10 PBS pH 7.4 (1X) Life Technologies Cat# 10010049 Tris-buffered saline (10X) Thermo Scientific Cat# J60764 rProtein A Sepharose Fast Flow resin Cytiva Cat# 17127903 FreeStyle 293 Expression Medium Thermo Fisher Scientific Cat# 12338026 PierceTM Protease Inhibitor Tablets, EDTAfree Thermo Fisher Scientific Cat# A32965 5M Sodium chloride (500 mL) Corning Cat# 46-032-CV 1M Tris-HCl, pH 8.0 solution. (1 L, Sterile) Teknova Cat# T1080 Bovine Serum Albumin (BSA) Fisher (Rockland) Cat# NC0263771 Ampicillin Sodium Salt (Crystalline Powder) Fisher Scientific Cat# BP1760-25 Carterra LSA Sensor Chip (CMDP) Carterra Cat# 4282 Amine Coupling Kit Cytiva Cat# BR100050 BiacoreTM Maintenance Kit, type 3 Cytiva Cat# 29229054 Dialyzer Midi VWR Cat# 71506-3 Lauryl Maltose Neopentyl Glycol (LMNG) Anatrace Cat# NG310 Penicillin-Streptomycin (10′000 U/mL) Thermo Fisher Scientific Cat# 15140122 L-Glutamine (200 mM) Thermo Fisher Scientific Cat# 25030081 Dulbecco’s Modified Eagle Medium (DMEM) Thermo Fisher Scientific Cat# 10313039 Heat Inactivated FBS Omega Scientific Cat# FB-02 (Continued on next page) Cell Reports 45, 117180, April 28, 2026 23 .. REAGENT or RESOURCE SOURCE IDENTIFIER FuGENE 6 Transfection Reagent FuGENE Inc. Cat# F6-5000 DEAE-Dextran Thermo Fisher Scientific Cat# 9015-73-0 Trypsin-EDTA (0.25%), phenol red Thermo Fisher Scientific Cat# 25200056 1X Dulbecco’s Phosphate-Buffered Saline (DPBS) Corning Cat# 21-031-CV Streptavidin AF647 Invitrogen Cat# S11223 Streptavidin-AF488 Invitrogen Cat# S21374 Streptavidin-BV421 Biolegend Cat# 405225 BD FC Beads BV510 BD Biosciences Cat# 661628 BD FC Beads APC-H7 BD Biosciences Cat# 661621 BD FC Beads APC-Cy7 BD Biosciences Cat# 661622 BD FC Beads PE BD Biosciences Cat# 661616 BD FC Beads PerCP Cy 5.5 BD Biosciences Cat# 661619 BD FC Beads BV786 BD Biosciences Cat# 661630 BD FC Beads PE Cy7 BD Biosciences Cat# 661617 BD FC Beads FITC BD Biosciences Cat# 661615 BD FC Beads APC BD Biosciences Cat# 661620 BD FC Beads BV421 BD Biosciences Cat# 661627 BD Resuspension Buffer (35mL) BD Biosciences Cat# 661614 Spherotech Anti-Human IgG/Protein Beads Spherotech Cat# HUP1505 Anti-Mouse Ig, κ/Negative Comp Beads BD Biosciences Cat# 552843 Mouse anti-human IgG BD Biosciences Cat# 555784 UltraComp Beads Plus Compensation Beads Thermo Fisher Scientific Cat# 01-333-42 ArC Reactive Beads Invitrogen Cat# A10346 Biotin Polystyrene Particles Spherotech Cat# TP-30-5 Glycerol, molecular grade Ricca Chemical Company Cat# 3290-32 Buffer EB Qiagen Cat# 19086 Ethanol, 100 Proof Pharmco Cat# 111000200 SPRI Beads Beckman Coulter Cat# B23319 Dynabeads Invitrogen Cat# 37002D Ambion Nuclease-free Water Invitrogen Cat# AM9937 RPMI Gibco Cat# 61870-036 EDTA Invitrogen Cat# 15575-038 CS&T Beads BD Biosciences Cat# 661414 Accudrop beads BD Biosciences Cat# 661612 Sheath Fluid BD Biosciences Cat# 99-30211-00 High Sensitivity D5000 ScreenTape Agilent Cat# 5067-5592 High Sensitivity D5000 Reagents Agilent Cat# 5067-5593 High Sensitivity D1000 ScreenTape Agilent Cat# 5067-5584 High Sensitivity D1000 Reagents Agilent Cat# 5067-5585 PhiX Control Library NextSeq V3 Illumina Cat# FC-110-3002 NextSeq 2000 P3 Reagents (100 Cycles) Illumina Cat# 20040559 Bovine Serum Albumin 30% Solution Fisher (Rockland) Cat# NC0263771 Microdialysis Plate 48 Wells 1 mL Thermo Fisher Scientific Car# A50466 Deposited data BG505 MD39 + RM19R Fab + 1–23 Fab model PDB: 9PIT This manuscript. (Continued on next page) 24 Cell Reports 45, 117180, April 28, 2026
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